Audience this guide is intended for pr incipal investigators and labora tory staff who conduct allelic discrimination assays using the 7900ht fast system. User interchangeable thermal cycling block formats let you select the format thats right for your project, using industrystandard 96well and 384well formats, as well as a novel 384well taqman low density array and a new fast 96well block that. Brevision date5 may 2012 this guide contains the information needed to prepare your site for installation of the applied biosystems quantstudio12k flex realtime pcr system quantstudio 12k flex system. User manual, getting started manual applied biosystems 7900ht user manual 384 pages fast realtime pcr system and sds enterprise database. The overall size and footprint of the abi 7500 was made more compact. Applied biosystems 7900ht fast realtime pcr system software download. View and download applied biosystems 7300 installation and maintenance manual online. Applied biosystems 7900ht user manual pdf download manualslib. Working applied biosystems abi 7900ht fast real time pcr system.
This small but robust pcr takes up less space than the applied biosystems 7900ht. Applied biosystems 7900ht fast realtime pcr system user. The running royalty component of that license may be purchased from applied biosystems or obtained by purchasing authorized reagents. For detailed instructions, refer to the full product manual. In realtime pcr, cyclebycycle detection of accumulated pcr product is possible by combining thermal cycling, fluorescence detection, and application specific software. Applied biosystems 7900ht fast realtime pcr system allelic. Select the data collection tab, then verify the default settings. Applied biosystems 7500 getting started pdf download manualslib. Applied biosystems 7900ht system spectral calibration kit. View and download applied biosystems 7500 getting started online. The applied biosystems microamp optical 384well reaction plate with barcode is designed to provide unmatched temperature accuracy and uniformity for fast, efficient pcr amplification. This instrument is also an authorized thermal cycler for use with applications licenses available from applied biosystems.
Fast realtime pcr system with computer available and configured for abi 7900ht abi 7900ht. This real time pcr machine is user friendly and does analysis for you too. Applied biosystems 7900ht fast realtime pcr system maintenance and troubleshooting guide pn 4365542 applied biosystems 7900ht fast realtime pcr system site preparation guide pn 4351923 abi prism 6100 nucleic acid prepstation users guide pn 4326242 dna isolation from fresh and frozen blood, tissue culture cells, and buccal. Five user attention words appear in the text of all applied biosystems user documentation. There are applied biosystems pcr plastics that are just right for your quantstudio realtime system, too. Applied biosystems 7900ht fast realtime pcr system from. Abi prism 7900ht sequence detection system user guide. Applied biosystems 7900ht fast realtime pcr system relative.
This is the users manual for the abi prism 7900ht sequence detection system. The applied biosystems 7900ht fast realtime pcr system is a realtime thermal cycler covered by us patents and corresponding claims in their nonus counterparts, owned by applied biosystems. This system is a sophisticated platform, applied biosystems 7900ht fast realtime pcr system user manual and 7500 fast realtime pcr systems this manual is written for principal investigators and. Realtime pcr systems chemistry guide content delivery. Sds softwareused for ct determination in realtime assays. Quantstudio 12k flex realtime pcr system as well as. Applied biosystems 7900ht fast realtime pcr system 6 user bulletin d. The abi 7500 realtime pcr system is a powerful platform for labs that require superior performance and maximum dye diversity. Applied biosystems power sybr green pcr master mix 1 x 5ml. The applied biosystems 7500 fast realtime pcr system represents the latest innovation in realtime technology fast thermal cycling, delivering highquality results in as little as 30 minutes. Applied biosystems 7500 realtime pcr system biocompare. No right is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to apparatus. The applied biosystems 9800 fast thermal cycler is an integral component of the applied biosystems 9800 fast pcr system, which reduces pcr reaction time from 2 hours to as little as 25 minutes. The applied biosystems 7900ht fast realtime pcr system employs the automation controller software for automated operation of the 7900ht instrument.
The automation accessory combined with 384well plate and 7900ht micro fluidic card capability make the 7900ht system ideally suited to meet the highthroughput requirements of todays drug discovery process. It can measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination snp genotyping assays and score the presence of gene sequences. If you use the primer express software to design probes and primers for your snp genotyping assay, follow instructions in the taqman universal pcr master. The applied biosystems proflex pcr system combines flexible configuration and control features to fit how. Sequence detection system is a highthroughput realtime pcr system that detects and qantitates nucleic acid sequences. View online or download applied biosystems 7500 fast installation and maintenance manual, getting started. Applied biosystems 7500 realtime pcr system applied biosystems 7500 fast realtime pcr system applied biosystems 7900ht fast realtime pcr system sds software v2. Applied biosystems 7900ht realtime qpcr system used. Applied biosystems abi 7900ht fast real time pcr system click on images for larger view here is a good. General description the applied biosystems 7900ht fast realtime pcr system is the only realtime quantitative pcr system that combines 96 and 384well plate compatibility and the taqman low. Quantitative detection of nucleic acid sequences using realtime analysis qualitative detection of nucleic acid sequences using end point and dissociationcur ve analysis for more information on the 79 00ht fast system, refer to 7900ht fast realtime pcr system online help. See the table below to find more documentation and their associated. Applied biosystems 7900ht fast realtime pcr system user manual and 7 500 fast realtime pcr systems this manual is written for principal investigators and with the 7500 fast realtime pcr system from applied biosystems.
Microamp optical 384well reaction plate with barcode. Applied biosystems abi prism 7900ht sequence detection system user s manual. The applied biosystems abi prism 7900ht fast realtime pcr system is a realtime quantitative pcr system compatible with 96 and 384 well plates. Applied biosystems quantstudio 12k flex realtime pcr. Abi applied biosystems 7900ht fast realtime pcr system. Allelic discrimination introduction getting started guide. View and download applied biosystems 7900ht user manual online.
The 7500 fast dx realtime pcr instrument delivers the performance required for highquality results in a 96well format. The abi 7500 is the predecessor of the abi 5700, made as the lower throughput version of the abi 7900ht. Applied biosystems 7900ht fast realtime pcr system 384well or 96well used unit is in good working condition, please see photos for details on cosmetic condition and whats included. Applied biosystems 7900ht system spectral calibration kit, fast 96well easytouse, preloaded, 96well optical reaction plates enable quick and easy calibration of the 7900ht fast realtime pcr system. Abi prism 7000 7700 7900ht, applied biosystems 7300 7500 realtime pcr system, applied biosystems 7500 fast realtime pcr system mx3000p stratagene lightcycler roche thermal cycler dice realtime system tp800, takara bio, japan 96well pcr plates and 8well pcr strips that are compatible with your pcr thermocycler. Applied biosystems abi prism 7900ht fast realtime pcr system.
Applied biosystems 7900ht fast realtime pcr system 7900ht fast system. Manuals and user guides for applied biosystems 7900ht. Microamp for use with equipment 2720 thermal cycler, 3500xl dx genetic analyzer, 7000 system, 7300 system, 7500 fast system, 7500 system, 7900ht fast system, 7900ht system, ab 2720 thermal cycler, geneamp 9700, quantstudio, simpliamp thermal cycler, steponeplus, veriti dx. Performing fast gene quantification pn 4352533 rq manager software user guide pn 4351670 snp manager software user guide pn 4351671 primer express software version 2. Unmatched speed and performance reproducible, specific and sensitive pcr results in just 25 minutes. Applied biosystems 7300 real time pcr system 96 well qpcr. This system is a sophisticated platform, application bio system 7900ht high speed realtime pcr system user manual, 7500 high speed realtime pcr system this manual is written for the chief researcher. Applied biosystems stepone and plus real time pcr systems user manual 8 4pack applied biosystems stepone and plus real time pcr systems user manual. Microamp for use with equipment 2720 thermal cycler, 3500xl dx genetic analyzer, 7000 system, 7300 system, 7500 fast system, 7500 system, 7900ht fast system, 7900ht system, ab 2720 thermal cycler, geneamp 9700, quantstudio, simpliamp thermal cycler, steponeplus. Abi 7900ht applied biosystems 4330966 fast realtime pcr. May 10, 2005 the applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time. Applied biosystems 7500 fast realtime pcr system contact information life technologies 850 lincoln centre drive foster city, ca 94404 poc. We also have applied biosystems assays and master mixes that support a variety of applications to fit your needs. Oct 03, 2016 abi 7900ht thermo scientific 4329001 fast realtime pcr 4330966 w stacker 11291 duration.
The 7900ht fast realtime pcr system is the only realtime quantitative pcr. Select the ramp rate tab, then verify the default settings. Two user attention words appear in applied biosystems user documentation. Relative quantitation using comparative c getting started. Description the 7900ht fast realtime pcr system is a realtime quantitative pcr system that combines 96well and 384well plate. Applied biosystems 75007500 fast thermo fisher scientific. Fast real time pcr system and sds enterprise database.
Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Applied biosystem abi prism 7900ht sequence detection system. Open the help from within the software by doing one of the following. Detailed info and photos for this exact instrument can be found on our website at pcr pcr system abi 7900ht applied biosy. Check out the most popular applied biosystems products for genetic analysis. Abi prism 7900ht sequence detection system users manual. Applied biosystems 7300 real time pcr system passes system checks and diagnostics.
This plate, constructed from a single rigid piece of polypropylene in a 384well format, is designed to work with applied biosystems 384well realtime pcr. The 7900ht system is a versatile research tool that can accommodate any realtime pcr need. This abi prism 7900 ht sequence detection system base unit, serial. This is the user s manual for the abi prism 7900ht sequence detection system. The plexor hy system af is a novel realtime pcr system for quantification of total human autosomal and human male y dna within a sample. We have 2 applied biosystems 7900ht manuals available for free pdf download. The abi 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual. Includes 384 well block, power cord and an ibm thinkcenter pc computer with sds v2. Applied biosystems 7900ht fast realtime pcr system and. The applied biosystems 7900ht fast realtime pcr system 7900ht fast system use s fluorescencebased pcr chemistries to provide. Applied biosystems 7900ht user manual pdf download. Role of xenobiotic metabolizing cytochrome p450s article snippet. Configuring the the software coordinates the action of the 7900ht instrument, the bar code reader, software and the plate handler while acquiring and saving raw data during each run. Plexor hy system for the applied biosystems 7500 and 7500.
Viia 7 realtime pcr system quick reference guide note. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems. For every chemical, read the sds and follow the handling instructions. The abi 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. This real time pcr system combines pre boxed or custom assays, and advanced thermal cycling systems with responsive software for a vast array of applications. Wear appropriate protective eyewear, clothing, and gloves. The 7900ht fast realtime pcr system is the only realtime quantitative pcr system that combines 384well plate compatibility with fully automated robotic loadingand now also offers optional fast realtime pcr capability.
The abi 7300 is great quantifying nucleic acid sequences without compromising data. Applied biosystems quantstudio 12k flex realtime pcr system. Applied biosystems 7900ht fast realtime pcr system and sds. Realtime pcr system supported software versions file extension applied biosystems 7900ht fast real. This technical manual describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime. No right is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to. Certain applied biosystems pcr reagents are developed and manufactured by roche molecular systems, inc. Applied biosystems 7900 ht fast real time pcr 384well. For safety and biohazard guidelines, refer to the safety appendix in the applied biosystems viia 7realtime pcr system user guide pn 4442661. We have 2 applied biosystems 7500 fast manuals available for free pdf download.
Abi applied biosystems 7900ht fast realtime pcr system thermo. Applied biosystems microamp 96well support base life. Applied biosystems offers a family of realtime pcr instruments with the 96well block format. Applied biosystems 7900ht fast realtime pcr system thermo. Complete the plate document setup as described in the applied biosystems 7900ht fast realtime pcr system and sds enterprise database user guide. The applied biosystems 7900ht fast realtime pcr system is a realtime thermal. The system is intended for laboratory analysis and is. User guide applied biosystems quantstudio 12k flex realtime pcr system site preparation publication part number4470654 rev. The 7900ht fast realtime pcr system is a realtime quantitative pcr system that combines 96 and 384well plate compatibility and the taqman low density array with fully automat ed robot ic loadingand now also offers optional fast realtime pcr capability. Applied biosystems 75007500 fast realtime pcr system getting started guide for genotyping experiments ix obtaining information from the software help the 7500 software help describes how to use each feature of the user interface. This qpcr is set up with the abis fast block that can provide results in as little as. The applied biosystems 7500 fast real time pcr offers an intuative and flexible solution for rapid detection and quantification of nucleic acids. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcr from sample prep to data analysis. Manuals and user guides for applied biosystems 7500 fast.
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